Insights & Perspectives
The following are additional insights and perspectives provided by those individuals who participated in this study. The participants were asked to answer the following question: "How would the use of the “next-generation” conjugated antibodies (i.e., antibody conjugates coupled to nanoparticles) help your research?"
In order to maintain their anonymity, individual comments are paired with the participant's Username, Job Position and Geographic Location only. BioInformatics has minimized the "cleaning/normalization" of these comments in order to preserve the integrity and flavor of the respondent's original thoughts and perspectives.
"Next generation" antibodies, if properly used, could form the backbone of biological research in coming years.
Devanjan, Post Doctoral Fellow, North America
"Next-generation" conjugated antibodies would allow me to extract more precise results that could help to speed up my research processes and assist in expediting a streamlined research facility.
Evan, Physician, North America
"Next-generation" experiments to match advantages of antibodies coupled to nanoparticles (e.g. paramagnetic beads) can exploit improved versatility in methods to sort cells and molecules. Other nanoparticles (e.g. quantum dots) can provide fluorescent stability that is advantageous in more complex biological situations, especially cell-cell interactions and the fate of transplanted cells. Combined, one can anticipate improved methods for isolating specific subsets of cells from a tissue and subsequently gain an improved understanding of how different cells modify each other's behavior in multicellular environments.
Jorge, Principal Investigator, Europe
“Next-generation” antibodies are unlikely to help me very much in Western Blots or affinity chromatography.
Hans-Ulrich, Lab Director/Supervisor/Coordinator, Europe
1.Devising new targeted drugs 2.Use as probes for bio-imaging/localization 3. Use as general biological tags 4.Use for more array/multiplexed assays.
Kamal, Post Doctoral Fellow, Europe
Above all, it will allow rapid and highly accurate identification of target molecules, including accurate diagnostics of infectious diseases, human diseases but also other research applications and rapid assay development.
Jamie, Principal Investigator, North America
Accelerate the pace we can isolate stem cells for culture and characterization.
Derek, Principal Investigator, North America
Allow tracking of viral particles on a flourometer.
Gabrielle, Principal Investigator, North America
Although gel excision is readily accepted, yields are variable, and the ability to purify proteins from particular cells would be enhanced with nanoparticles. Anyone who compares disease progression is always concerned with a dilution effect if the disease progresses from 10% of the whole tissue affected to an increasing number of cells expressing some "disease specific genes" (as opposed to immediate and late genes).
Brian, Principal Investigator, North America
Although maintaining the costs, next-generation antibodies would provide quantitative measurements for Western-blotting and reproducible FACS.
Lisardo, Lab Director/Supervisor/Coordinator, Europe
Antibodies conjugated with nanoparticles may be used together with laser activation for specific destruction of cell compartments or structures. Using this method, it is necessary that the antibody specifically bind to the target, however, it is not relevant, e.g. in case of a receptor, that the antibody binds to the recognition site. This significantly improves efficacy and reliability of such receptor blockades.
Gernot, Principal Investigator, Europe
Antibodies coupled to dendromeres.
Michael, Professor/Teacher, North America
Antibodies have their limits, especially the ability of primary antibodies to specifically/uniquely recognize an epitope. That has little to do with how you label/with what you couple the antibodies to.
Dubravko, Graduate Student/Research Assistant, Europe
Antibodies should be designed based on the demand, which depends on the questions you want to ask. In the future, if I do not need antibody conjugates coupled to nanoparticles, then their creation will be of no use to me. Maybe others will need it.
Amrisha, Post Doctoral Fellow, North America
Antibodies specific for cancer cells, coupled to anticancer drugs, may help with site-specific transport of drugs.
Daniel, Professor/Teacher, Europe
Antibodies that are widely used for traditional biological research will find their way for newly developed proteomics in the post genomics. All the antibodies used in proteomics will be labeled with Cy3 or Cy5, and the corresponding secondary antibody or substrate systems should be developed in order to use all these antibodies.
Mingang, Staff Scientist, North America
Antibody conjugates coupled to magnetic nanoparticles have helped my research in enabling the positive and negative selections of various cell subpopulations, such as the enrichment of rare adult stem cells from bone marrow.
Karen, Principal Investigator, North America
Antibody conjugates coupled to nanoparticles for designing a diagnostic kit.
Yuri, Post Doctoral Fellow, North America
Antibody conjugates coupled to nanoparticles may help me in identifying the subcellular localization of proteins.
Jian, Post Doctoral Fellow, North America
Antibody conjugates coupled to nanoparticles will open a new era of multiplex antibody-based assay development. However, these reagents will still only be as good as the starting antibody.
Scott, Lab Director/Supervisor/Coordinator, North America
Antibody nanoparticle conjugates open up the potential for limitless analysis of cell surface protein expression by flow cytometry from a single tube.
Greg, Lab Director/Supervisor/Coordinator, North America
As a drosophila researcher, I find it extremely difficult to find antibodies that are either drosophila specific or cross reactive with drosophila. Cell signaling seems to have the highest number of useful antibodies for my research. As a recommendation to other antibody suppliers, they need to have a higher selection of different species for different genes, and in the case of Santa Cruz....THEY NEED TO TEST THEIR ANTIBODIES BEFORE THEY SELL THEM!!!!!!!
Jill, Laboratory Technician, North America
As an educator, I place great importance on the ability of all bioscience products to perform reliably every time. Though not all experimental protocols work as predicted owing to technique, experience, and the quality of supporting reagents, it is extremely important that my students achieve some measure of success in the lab. If "next-generation" conjugated antibodies increase the reliability of our protocols, they are more than welcome as valuable additions to our curriculum.
Karl, Professor/Teacher, North America
As I am working mainly in veterinary research, I doubt this will be useful to me at the moment.
Dirk, Principal Investigator, Europe
As we look for adhesion molecules, etc., I guess the use of nanoparticle conjugated antibodies would limit the side effects like activation on the cells. Therefore, I'd guess it was fine for us.
Heike A., Staff Scientist, Europe
At the current time, I have no idea about this kind of product's impact on my research.
Richard, Staff Scientist, North America
At the moment I don't expect to use these types of reagents, but if I were to use them, this would be to identify/characterize something really small!!!!!
Antonio, Post Doctoral Fellow, Europe
At the moment, probably they would not be useful. However I could envisage a time when we would want to access an area that perhaps only nanoparticles coupled to specific antibodies would be able to infiltrate without causing tissue damage.
Christine, Laboratory Technician, Europe
At the moment, this wouldn't help my research unless the equipment for detecting these nanoparticles became available at a reasonable cost. If there is reliable detection methods that gave great results, then it would be useful in the future.
Rachel, Post Doctoral Fellow, Europe
Batch processing of affinity chromatography step will become simple.
Mosuvan, Production/Manufacturing, Asia
Because of their very narrow emission spectra, nanoparticle conjugation to antibodies will allow a much greater degree of multiplexing in immunocytochemisty, flow cytometry, and high content screening, and accelerate drug discovery research.
Ronald, Staff Scientist, North America
Better localization within compartments on the cell surface.
Terrance, Principal Investigator, Australasia/Pacific
By allowing me to multiplex, they will give me more data per sample point.
Thomas, Lab Director/Supervisor/Coordinator, North America
By being able to more specifically locate a protein, I can advance my research and spend less time optimizing sub-standard, cross-reactive antibodies.
Eleanor, Post Doctoral Fellow, North America
By being flexible in specificity and usability.
John, Lab Director/Supervisor/Coordinator, Europe
By bringing me a step nearer to small-molecule, non-competitive immunoassay as well as bringing back immunoassay to the mainstream of bioanalysis — e.g. for biomarkers and small molecules.
Richard, Lab Director/Supervisor/Coordinator, Europe
By conjugating novel fluorophores to fragments of high quality antibodies, it might be possible to bypass resolution thresholds with which we are recently dealing. New two-photon emitters will in combination with revolutionary 4-Pi microscopy remodel the standard image techniques. Microscopy was yesterday — tomorrow is Nano-Bio-Photonik!
Jens, Graduate Student/Research Assistant, Europe
Call it confidence, perhaps naiveté, I just go for BD every time.
John W., Lab Director/Supervisor/Coordinator, Australasia/Pacific
Cell signaling pathways, cell death and cell survival in health and diseases.
Pushpa, Principal Investigator, North America
Conjugated/labeled antibodies have great potential in the diagnosis and treatment of the various diseases. Nanoparticle conjugated/radiolabeled antibodies would be useful for the treatment and diagnosis of cancer in near future.
Subhash, Post Doctoral Fellow, North America
Coupled to highly specific antibodies, this would allow multiplexed analysis of signaling pathways using automated imaging systems that would provide more spatial/temporal understanding of cell signaling.
Keith, Staff Scientist, North America
Currently, we have no need for this technology.
David, Lab Director/Supervisor/Coordinator, North America
Determination of drug delivery and oral bioavailability and ability of the nanoparticle/drug complex to cross the blood brain barrier.
Greg, Principal Investigator, Australasia/Pacific
Diagnostic, treatment (experimental animal models), magnetic nanoparticles for treatment and monoclonal antibodies.
Monica, Pharmacist, North America
Dye-Doped Silica Nanoparticle-Antibody Conjugates will enhance the quality of sensitive pathogen detection methods.
Herman, Principal Investigator, Europe
Each company seems to have their specialty. I used to buy all sorts of signaling Ab from Upstate, and when Cell Signaling came along, I never turned back to Upstate, except for their phosphorylated Smad1 Ab. Cell Signaling's Ab are much more superior in quality. However, for secondary Ab, I would not buy from any vendor except from Jackson. They have the best complete line of secondaries. Specificities, pre-adsorbed, or types of conjugation...nobody can beat them. They have been around for a long time, and I had never had ANY trouble with their Ab...always work the first time, and work the same every time. And then, there is Santa Cruz. I tried and I don't think I will buy another Ab from Santa Cruz, enough said. It's a gimmick. R and D is best for neutralizing Ab. Each company really has their own niche.
Lillian, Lab Director/Supervisor/Coordinator, North America
Extremely... we are needing a highly efficient, low background, bright and scannable detection of a rare event... nanoparticles may be the thing for which we've been waiting patiently.
Morry, Professor/Teacher, North America
For cell separations of mixed primary cultures using magnetic of FACS technologies.
Jason, Lab Director/Supervisor/Coordinator, North America
For high throughput microchip protein assays.
Julie, Post Doctoral Fellow, North America
For immunoelectron microscopy, nanogold-conjugated secondary antibodies have been successfully used especially for the preembedding techniques. Additional nanoparticles that can be used for either scanning or transmission electron microscopy would be very helpful.
Chang-Sub, Professor/Teacher, Asia
For my research, antibody conjugates coupled to nanoparticles might serve as a valuable tool to evaluate the in vivo tumor targeting potential of the anti-mucin antibodies we have developed. Such conjugates would be useful alternative to the radiolabled antibody biodistribution studies. We can use antibody conjugated to gold nanoparticles to visualize antibody localization in respected tumors from animals following iv injection. Additionally, antibodies coupled to iron oxide nanoparticles can be used for MR imaging to localize the tumors and remote metastatic sites.
Maneesh, Post Doctoral Fellow, North America
For my research, the application of antibodies specific for various post-translational modifications (sumoylation, phosphorylation, ubiquitinilation, etc.) conjugated to a series of fluorescent dyes to allow true multi-parameter analysis of the activation state of various signaling intermediates in a single sample would mean a great leap forward.
Martijn, Post Doctoral Fellow, Europe
For protein chip analysis, it's maybe the most important thing.
Yu, Graduate Student/Research Assistant, Asia
For studies "in vivo" for bioengineering to influence processing.
Richard, Principal Investigator, North America
Generate a revolution in cellular & sub-cellular protein profiling.
Muthuswamy, Staff Scientist, Asia
Great diagnostic and therapeutic applications potential, more refined ultra-structure studies.
Arseni, Post Doctoral Fellow, Europe
Help to discover the detailed mechanisms of cell to cell signaling in systemic inflammation.
Christian, Professor/Teacher, Europe
Helpful but not very.
Burra, Professor/Teacher, North America
Helpful in design of innovative experiments.
Andrew, Principal Investigator, Australasia/Pacific
Hopefully nanoparticles will enable the development of more effective high throughput screening of primary hybridoma clones using live cell targets.
Luis, Production/Manufacturing, North America
Hopefully they will enable faster and simpler detection as well as automation.
Yoram, Post Doctoral Fellow, North America
Hopefully they would allow me to use immunofluorescent techniques without the problems of photobleaching and depletes the need for different excitation filters.
Lynn, Post Doctoral Fellow, Europe
I am using conjugated antibodies in my research to find a for cure for AIDS and various other viruses. I have made some breakthroughs and plan on continuing my studies.
Kristen, Administrator, North America
I am very sorry to say, that I have not much information regarding such antibodies. So I can not make a statement about it.
Veronica, Graduate Student/Research Assistant, North America
I do not think "next-generation" antibodies are a product everybody is going to use in everyday lab work. They might be of great value for specific purposes, but the term "next-generation" implies some kind of revolution in my work-flow (which is not going to happen).
Michael, Post Doctoral Fellow, Europe
I don't envision nanoparticle-conjugated antibodies being important to my research in the foreseeable future.
Todd, Staff Scientist, North America
I don't plan to use antibodies conjugates coupled to nanoparticles in the immediate future.
Jay, Professor/Teacher, North America
I don't plan to use antibodies conjugates coupled to nanoparticles in the immediate future.
Francisca, Principal Investigator, North America
I don't plan to use antibodies conjugates coupled to nanoparticles in the immediate future.
Nicola, Professor/Teacher, Europe
I don't plan to use antibodies conjugates coupled to nanoparticles in the immediate future.
Joanna, Staff Scientist, Europe
I don't plan to use antibodies conjugates coupled to nanoparticles in the immediate future.
Mohanish, Lab Director/Supervisor/Coordinator, Asia
I don't plan to use antibodies conjugates coupled to nanoparticles in the immediate future.
Radhika, Post Doctoral Fellow, North America
I don't plan to use antibodies conjugates coupled to nanoparticles in the immediate future.
Sheldon, Lab Director/Supervisor/Coordinator, North America
I don't plan to use antibodies conjugates coupled to nanoparticles in the immediate future.
John, Staff Scientist, North America
I dream of antibodies-based tools that offer the capability of tracking several molecules within living cells at the same time. Specifically, testing molecular trafficking, interactions and signaling at the same time, in the same cell. We could imagine developing antibodies that still have a biological activity while being conjugated; antibodies that can enter the cell without damaging it. This is important for those who work on human disease and have little material for investigations.
Veronique, Post Doctoral Fellow, Europe
I have been using Quantum Dots in a number of pilot studies to determine their applicability for multiplexed drug delivery systems.
Todd, Lab Director/Supervisor/Coordinator, North America
I have yet to look into this.
Christine, Professor/Teacher, North America
I hope that these antibodies will enhance sensitivity. It would be nice to detect few antibodies bound just by repeated or prolonged incubation with the color substrate.
Dirk, Lab Director/Supervisor/Coordinator, Europe
I know virtually nothing about nanoparticles. First, tell me what they could do and then I can tell you how they might help my research.
Jeff, Graduate Student/Research Assistant, North America
I plan to use the "next-generation" conjugated antibodies for cell separation, identification and quantification.
Palat , Professor/Teacher, Asia
I see more sense in antibody arrays then in antibodies coupled to nanoparticles. However, the technology must gain a lot in terms of stability, specificity and the ease of use to deliver promising results.
Sergei, Staff Scientist, North America
I see multiplexing as the way forward with panels of reagents. This will enhance the speed and breadth of our analysis. I see this in both protein arrays and automated screening (e.g., immunohistology).
Neil, Principal Investigator, Europe
I think that is a new field in laboratory research, especially for biomolecular investigation.
Giuseppe, Professor/Teacher, Europe
I think that it would be very applicable to our macular degeneration and glial scarring studies, especially with regard to the mechanism underlying these events.
Linda, Principal Investigator, North America
I think the use of conjugated antibodies has been limited by the means of detection for the conjugate. With the advent of nanoparticles, detection limits may be lowered further and that is the main advantage I foresee from the next generation of antibodies.
Dany, Lab Director/Supervisor/Coordinator, North America
I think there are enough problems with conventional secondary conjugates that consume my time and need to be worked out. Additional technologies without benefit in standard procedures is beyond the limits of many labs.
Michael, Principal Investigator, North America
I think they may help greatly in our research to develop vaccines and anticancer strategies. For instance, nanoparticles carrying cytokines conjugated to convenient antibodies may help to target those to specific tissues and cells.
A., Professor/Teacher, Europe
I think they would optimize the procedure and in the end they may prove to be far more specific in detections then those used so far.
Emil, Professor/Teacher, Europe
In my experience (and I'm not alone, from talking to colleagues) quite often they don't work. Santa Cruz is particularly awful in this respect. I have spent so much money with them over the years on what turned out to be pots of dirty PBS, devoid of any discernable reactivity with the molecule on the tube label. My heart sinks when the only antiserum I can find to a protein of interest is a Santa Cruz goat polyclonal. Odds are it'll be a waste of time and money.
Paul, Principal Investigator, Europe
I was not aware that "Next-generation" conjugated antibodies even existed. It's an interesting idea. I need to know more about their capabilities.
Michael, Quality Assurance/Quality Control, North America
I will apply the antibodies to cells in culture and track them with the use of nanoparticles using time lapse, confocal microscopy and transmission electron microscopy and I bet I could used them with scanning electron microscopy.
Ivan, Professor/Teacher, North America
I work on several biosenors with the potential to replace the current state of the art diagnostic processes. Nanoparticles are essential in this type of research. I currently use colloidal gold, fluorescent, and latex nanoparticles.
Tom, Department Head, North America
I would like to use them especially for cellular localization studies and protein modifications as most of the fluoro-labeled systems do not have enough sensitivity and specify for these approaches and I have to label cellular organelles as well.
Zgr, Graduate Student/Research Assistant, Europe
I would not because I confidently predict that aptomer technology is cheaper than antibody technology with added bonus related to size and delivery for nanoparticles.
Ken, Professor/Teacher, Europe
I would rate these very highly, but I feel there is always a barrier, related to price and experience, against moving on to the next generation of products. I am still using good old DAB staining rather than fluorescent probes, because I don't have instant access to a fluorescent microscope. The post grad students also find it easier to start with the older methods when training. Once any new technology has been tried and shown to be superior, I will start to use it, but I let others do the initial legwork.
Jean, Professor/Teacher, Australasia/Pacific
I would use it for microarray analysis to evaluate protein expression.
Joao, Lab Director/Supervisor/Coordinator, Europe
I would use the next-generation conjugated antibodies for "real-time" isolation of autologous adult pluripotent stem cells for transplant.
Henry E., Principal Investigator, North America
I would use these antibodies for immunohistochemical labeling of proteins for analysis at the electron microscopic level. The smaller size of the particles would allow the antibodies to penetrate deeper into the tissue, allowing for better signal detection.
LeeAnn, Post Doctoral Fellow, North America
I would use these antibodies in bioengineering experiments.
Suchitra, Post Doctoral Fellow, North America
Ideas not antibodies could help my research.
Alexander, Lab Director/Supervisor/Coordinator, Europe
If I understand the concept correctly, if would help a lot. For Flow cytometry, when you are doing multicolor experiments, you need many options for conjugating primary and secondary antibodies, especially for fairly rare antigens.
Kari, Laboratory Technician, North America
If my current research proposals are funded, next-generation conjugated antibodies could be feasible in the *next* round of funding. I would say that my need for next-generation conjugated antibodies would be a minimum of five years down the road.
Jen, Post Doctoral Fellow, North America
If such antibodies can be made dependably visible in the transmission electron microscope, this would remove a major stumbling block in immunoelectron microscopic research and interpretation, since one would not be depending necessarily on secondarily labeling antibodies with a reagent such as Protein A-gold. In addition, permeabilization of free-floating sections and subsequent infiltration with these conjugated antibodies might prove to be more successful in penetrating to significant depths; at present, even immunoperoxidase methods are only able to extend to ca. 9 µm or less, thus making interpretation of ultra thin sections prepared from such thicker slabs (50 µm usually) undependable (i.e., the thin sections must be taken at the very surface, and the lack of reactivity cannot necessarily be interpreted as being from lack of antigen, but could also be because of failure to penetrate to the depth at which the section is taken).
Michael, Laboratory Technician, North America
If the antibody conjugates are fluorescent and functionalized, coupling them to metallic nanoparticles helps us to employ near-field optical spectroscopy and trace their motion inside the cells. Thus, we have a cell-tracking device that can differentiate between healthy and diseased cells by the differential affinity of these cells to the fluorescent antibody molecule.
Aravind, Principal Investigator, North America
If the conjugated antibodies are coupled with devices that can handle high throughput detection, they could revolutionize current assay platforms. An antibody-based assay, such as FP, is frequently limited by the fluorophore due to several factors including interference by colored compounds in the screening library. If the "next-generation" conjugates can eliminate this and other types of limitations and maintain a homogeneous "mix and measure" assay, the impact on HTS screening should be dramatic.
David, Staff Scientist, North America
If the nanoparticles can be induced to emit light, I would use them in Flow cytometry. If the quantum yield is high and the emission spectra is narrow, it would benefit our research and increase the number of Abs that can used at time with the use of a single laser.
Lionel G, Professor/Teacher, North America
If this technology is easily adapted to already released (tried and tested) instrumentation then this technology would be highly advantageous.
Wanda, Staff Scientist, North America
I'm pretty happy with the results I've gotten so far, so the use of the "next generation" would depend on the performance/advantage vs. cost.
Randy, Post Doctoral Fellow, North America
Improve the ability to analyze complex mixtures of proteins during process development.
Ed, Production/Manufacturing, North America
In addition to the high stability and better rate of fluorescence of the nanoparticle-linked antibodies, one would expect to use them on living cells and so they can be used with new reagents that quickly and efficiently deliver antibodies directly into cultured cells (like the ones developed by ActivMotiv or PolyPlus) without any detergent. In that way we would get close to what GFP proteins gave us without the difficult cell line gene stabilization problems.
Christian D., Lab Director/Supervisor/Coordinator, Europe
In my mind, conjugated antibodies will play an increasing role in validation of new drug targets to further enhance the value in drug discovery.
Peter, Staff Scientist, North America
In theory, they would be a wonderful alternative to the current options, however, I have to see the data that demonstrates its performance.
Betty, Lab Director/Supervisor/Coordinator, North America
In vivo detection of labeled cells.
Tamas, Lab Director/Supervisor/Coordinator, North America
In vivo targeting.
Aiyappa, Principal Investigator, North America
Increased sensitivity through increased conjugate amplification would greatly increase my use of antibodies. Currently, antibodies are not sensitive enough for many of my interests, many of my protein concentrations are below the level of detection. Thus, I am forced to use RNA concentration and attempt to correlate it with protein expression.
Wayland, Staff Scientist, North America
It definitely increases the quality and reproducibility of research.
Bhavani, Graduate Student/Research Assistant, Europe
It depends by the performance of these reagents. At the moment I did not use one, therefore I do not know whether they will perform better that those available. Are they less toxic for in vivo studies? Do they have less problems about fading than fluorescent conjugates? Are they more sensitive and specific? Will they cost less? Are they simpler and less time demanding for using?
Pasquale, Lab Director/Supervisor/Coordinator, Europe
It depends on detection; wouldn't help much unless the conjugates can be used in our standard protocols.
Pradip, Staff Scientist, North America
It has made a world of difference in my studies. What used to take weeks, now can be done in days, even hours. It is easier and faster than when I first started out, and even more accurate. What a wonderful advance in science and medicine.
Brenda, Pharmacist, North America
It is crucial to move to studies of compartmentalization of signal transduction components in order to better understand how cross-talk and signal specificity are achieved. Use of nanoparticle conjugates offers a unique opportunity to spatially localize and follow signaling molecules through cellular compartments and to learn how cells are able to "compartmentalize" signaling proteins.
Heber, Professor/Teacher, North America
It is very important for the suppliers to provide the following : 1. Smaller pack sizes for labeled/conjugated antibodies 2. Custom combination of small pack sizes into convenient detection kits, for example, a kit for clonotyping T cells (V beta or V alpha repertoire).
Rahul, Post Doctoral Fellow, North America
It obviously depends on the nature of the nanoparticles. We currently use ligand targeted gene delivery systems for in vivo targeted gene delivery. Comparison of nanoparticles to our systems, especially if these had some reporter, such as a quantum dot, would aid in determining the effect of surface properties and particle size on specific and non-specific uptake.
Sean, Professor/Teacher, North America
It should be better if the conjugated antibodies have the function of second antibodies, which can help us save a lot of time and money to focus on our experiments.
Zhong-Min, Graduate Student/Research Assistant, Asia
It will be helpful!
Wu, Professor/Teacher, Asia
It will be most useful for my ongoing research.
Anjaneyulu, Graduate Student/Research Assistant, Asia
It will bring the immunofluorescent-oriented studies to a new dimension.
Ladislav, Principal Investigator, Europe
It will definitely enhance the research by adding more precise detection.
Navin, Staff Scientist, Asia
It will help for localization of proteins (and their interaction) inside the cell. Very useful tool for 3D electron microscopy.
Jean-Paul, Lab Director/Supervisor/Coordinator, Europe
It will help in the study of protein and cellular structures in vivo.
Stanislav, Staff Scientist, North America
It will improve precision.
Abayomi, Medical Technologist, Africa
It would ABSOLUTELY help my research. My research is moving the area of using nanoparticles and the availability is minimal.
Heather, Lab Director/Supervisor/Coordinator, North America
It would allow me to use less expensive, simpler to use, chip-based flow cytometry instrumentation because of the strong signals.
Marc, Staff Scientist, Europe
It would enable me to "multiplex" and be able to visualize different molecules at the same time.
Helen, Staff Scientist, North America
It would greatly help if the prices would come down! A lot!!!!!!! Also, another species (chicken? — more stable) would be great.
Vivienne, Professor/Teacher, North America
It would help a lot. A high specificity, no background, and a good rate of combination should be available.
Anja, Lab Director/Supervisor/Coordinator, Europe
Let's see what they will bring to us.
Gilbert, Professor/Teacher, Europe
Location of proteins within tissues and cells in response to a toxicological challenge.
Lou Ann, Principal Investigator, North America
Make it much easier.
Julie, Quality Assurance/Quality Control, North America
May reduce number of assay steps. May lower, background (noise) and increase signal. Availability of well standardized reagents).
Rishab, Lab Director/Supervisor/Coordinator, North America
Micro ferrous particles (which some would consider nanoparticles) are *already* being used routinely in our (and others') laboratories. We use them for magnetic separations of cells into various phenotypes. This plays a central role in the design of our research methods. I think it would be nice to have these ferrous particles themselves labeled with fluorochromes so that they could be followed by flow cytometry. Also, would it not be possible to include a detector on a cytometer that could pick up the magnetic flux associated with these particles so that no fluorochromes would be needed — sort of like a micro metal detector? Also: We frequently order large numbers of antibodies in a single order, so it was unclear as to whether the total number of antibodies ordered was the question, or the number of times we ordered antibodies.
David, Principal Investigator, North America
Monitoring of controlled processes is a key to functional analysis. In our project we hope to eliminate transport of immune-relevant molecules.
Norbert, Graduate Student/Research Assistant, Europe
Most of antibodies I have used are good, and I think the key of conjugated antibodies is their stability, especially for fluorescein.
Feng, Graduate Student/Research Assistant, Asia
Multilabel approaches and improved (linear) quantification.
Stephen, Staff Scientist, North America
Multiplexing protein research with the help of labeled antibodies would allow for faster data accumulation.
Krzysztof, Administrator, Europe
Multiplexing.
Haeri, Lab Director/Supervisor/Coordinator, North America
My "favorite" supplier is Rockland because of it's custom antibody service, which in our experience has been excellent. This type of service will grow in importance in the Pharma R&D sector because of the critical role of biomarkers for drug activity as new drug candidates move through clinical trials and into the marketplace. Particularly in the area of cancer therapy, FDA has taken the point of view that patient prescreening for probability of response to the drug (regardless of whether it is a biological, an antibody, or a small molecule). The most cost efficient and marketplace ready way to go about this is with immunoassay. In addition, data on performance of advanced technology approaches to biomarkers in clinical trials, e.g. maldi-tof and chip technology, does not yield the kind of positive predictive value for drug response that could justify the cost and technical challenge of these procedures. Immunoassay and PCR-based assays will reign for the next five years at least.
Robert, Lab Director/Supervisor/Coordinator, North America
My work is "in between" research, discovery, production and API qualification and release testing. I need often specialized product I have to custom make. For available reagent these companies are excellent in quality and customer satisfaction. I would like though to see more "GMP" qualification of their products.
Simon, Staff Scientist, North America
Nanodevices may make it possible to target antibodies and anticancer drugs to specific tumor locations in live tissue.
Thomas, Professor/Teacher, Europe
Nanoparticle antibody conjugates are under development in our lab for specific applications. They have specific advantages (e.g., new detection / separation methods, higher sensitivity) and disadvantages (e.g., internalization into living cells, non-specific binding of the nanoparticle). Future will show which applications will benefit from them!
Rolf, Principal Investigator, Europe
Nanoparticle conjugated antibodies would be very useful to me for delivering drugs/other compounds to specific cell types in the brain in the animal model of human disease that I work on. This would allow me to assess the effect of these compounds when they are targeted rather than working systemically. Because the signaling in the brain is so complex and because there are so many auto-feedback loops, targeted drug/compound delivery would assist me in parsing out these pathways.
Madhuri, Graduate Student/Research Assistant, North America
Nanoparticle conjugates will be used for antibody incorporation studies that address both viral neutralization and for membrane receptor trafficking studies where the smaller size will reduce transport interference.
C. David, Department Head, North America
Nanoparticle conjugates, such as those employing quantum dots, would allow me to image multiple antigens more effectively than can be done at present with fluorophore conjugated antibodies.
Ronald, Principal Investigator, North America
Nanoparticle-conjugated antibodies are useful in the treatment of various forms of cancer, but could be applied to developmental biology model systems to specifically target and manipulate cell signaling pathways in vivo throughout development.
Lars, Graduate Student/Research Assistant, Europe
Nanoparticles conjugated antibodies will change the way leading edge immunological research is conducted by way of its versatility and integrated process use. The technology by way of its multiple use promises to save the time in drug discovery & development research. Savings in terms of cost and time will give competitive edge to companies who will use such technologies for molecular medicine research.
Arkesh, Department Head, North America
Nanoparticles of what?! How much smaller than biotin or a fluorochrome does a moiety need to be? Since these have little effect on specificity or background, inventing new moieties seems more like advertising than science.
Michael, Post Doctoral Fellow, North America
Next generation antibodies would revolutionize the technologically driven science of this era.
Kiran, Post Doctoral Fellow, North America
Next-generation antibodies will improve the research productivity.
Shantha, Principal Investigator, North America
Next-generation conjugated antibodies will help us to study mechanisms in situ and follow pathways more easily.
Margaret, Post Doctoral Fellow, North America
Next-generation conjugates could be extremely valuable, providing that supplies offer sufficiently diverse options.
Leonard, Post Doctoral Fellow, Europe
Next-generation conjugates will help with my multiplexing needs for immunofluorescence and ELISA based assays. Since we are moving into high content based screens this would allow us to answer several questions at the same time and make do with a single assay for secondary assay in drug screening in drug discovery.
Malathi, Post Doctoral Fellow, North America
None.
Aaron, Lab Director/Supervisor/Coordinator, North America
Normalization and/or background control.
Cheng, Department Head, North America
Not at all.
Yongjian, Staff Scientist, North America
Not at this time.
Elaine, Staff Scientist, North America
Not especially: I need simple, reliable amplified immunoassay techniques that are relatively cheap, for quality control purposes. For this reason, chemiluminescence is as sophisticated as we need, and chromogenic Western blot and EIA are our staples.
Ed, Professor/Teacher, Africa
Not much at the moment as I do not know much about this technology.
Alexander, Lab Director/Supervisor/Coordinator, Australasia/Pacific
Not much right now, I am afraid.
Rachel, Lab Director/Supervisor/Coordinator, Africa
Not much.
Pu, Staff Scientist, North America
Not sure, but would like to use this powerful tool in future.
Satish, Post Doctoral Fellow, North America
Nothing at this time.
Vinny, Laboratory Technician, North America
Nothing.
Ingolf, Production/Manufacturing, Europe
Our projects vary a lot, so I can't really predict how these new antibodies would fit in our research scheme.
Luc, Staff Scientist, North America
Our research group primarily works on epithelial cells on signaling pathways from membrane to the nucleus involved with cell polarity. We need to see where proteins are compartmentalized in cells using confocal microscopy and TEM. The visualization of precise localization of proteins is often very difficult with fluorescence dyes. We hope the next generation nanoparticle-conjugated antibodies will help with our research.
Richard, Lab Director/Supervisor/Coordinator, Australasia/Pacific
Our research involves the foreign body reaction to materials used in the manufacture of medical devices for all uses i.e. long term implants, drug delivery and tissue engineering. We used an activated monocyte-derived macrophage cell system to study the foreign body reaction. By using antibody conjugated to nanoparticles we could observe which proteins/enzymes are unregulated or downregulated without interfering with the cell processes.
Rosalind, Principal Investigator, North America
Please give us some free sample or give us more discount when we want to buy many antibody at the small quantities.
Xiang, Post Doctoral Fellow, North America
Probably quite a lot.
Umberto, Lab Director/Supervisor/Coordinator, Europe
Provided the quality and specificity of the antibodies are high then nanoparticle conjugates are potentially very useful for increasing the resolution and precision of images and thus the information derived from image analysis.
Kim, Principal Investigator, North America
Quality is overall more important than anything else.
Giuseppe, Principal Investigator, North America
Quantum dot conjugated antibodies in particular will allow for the simultaneous imaging of multiple proteins in the cell without the complications of different excitation wavelengths and bleaching times experienced with traditional fluorophores.
Michael, Post Doctoral Fellow, North America
Quantum dot or other nanoparticle conjugated antibodies will increase the repertoire of available visualizing tools for fluorescence and confocal microscopy. Having a larger set of tools (colors) that are more reliable (low photo-bleaching/quenching) will make co-localization and real-time trafficking studies much easier and more robust.
Jim, Graduate Student/Research Assistant, North America
Quantum dots for flow cytometry and immunofluorescence.
Alvin, Professor/Teacher, Asia
Quite a lot, depending on the quality of the product, and availability.
Thomas, Staff Scientist, Europe
Real time microscopy to see transcription factors move to nucleus to bind DNA and possibly visualize multiple proteins (interactions?) at one site if nanoparticles can be distinguished from one another!
Robyn, Professor/Teacher, North America
Real-time protein detection/quantization in living cells (i.e., +/— stimulus).
Todd, Laboratory Technician, North America
Recent research (June 2004) at the Rice University in Houston shows that when nanoparticles of a certain composition are injected into a malignant tumor in mice and the tumor exposed to harmless infrared light, the nanoparticles heat up really fast and burn the tumor locally within minutes. So, it is not very difficult to imagine that in the near future, therapeutic antibodies tagged with such nanoparticles would deliver the nanoparticles into the desired area. The nanoparticles could be the kind described above or they could be catalytic devices that interact with proteins or DNA and show specific effects. They could also be drug delivery agents which deliver a labile drug wherever the antibody takes them. For the purpose of fundamental research, the same principles would apply, and using these unique reagents, a whole new range of research methodologies could be developed to understand biological functions at the molecular level. Since my current interests are signal transduction and transcriptional regulation, I could envision using catalytic nanoparticle tagged antibodies for specifically and effectively knocking out signaling molecules and transcription factors at a given time point. I could use tracer nanoparticles to trace the organ, tissue and cellular localizations of proteins on a real time basis. I could also envision numerous other uses like study of chromosome structure, protein-protein interactions and protein purification.
Vashisht, Post Doctoral Fellow, North America
Recombinant? Antibodies specific to small molecules and metabolites.
Wojtek, Lab Director/Supervisor/Coordinator, Australasia/Pacific
Since my research involves determining entry of filoviruses, it would be very useful for me to use secondary antibodies coupled to nanoparticles to track the trafficking of the virus through various intracellular compartments and determine the exact site of localization.
Suchita, Graduate Student/Research Assistant, North America
Since there's interest in nanoparticles as a means of drug delivery, it would be nice to know if the particles bind to blood components. This can simply be done by having the nanoparticle labeled.
Ginger, Staff Scientist, North America
Single molecule tracking/trafficking in real-time imaging.
Harry, Lab Director/Supervisor/Coordinator, North America
Studying the intracellular interactions and mobility.
Suresh, Professor/Teacher, Europe
Target 2 proteins at once of vary different sources.
Anne, Graduate Student/Research Assistant, North America
That will expand the capacity of flow cytometry, and may therefore help in some area of our research.
Liwu, Principal Investigator, North America
The "next-generation" antibodies may become very useful in flow cytometry.
Carolyn, Staff Scientist, North America
The "next-generation" conjugated antibodies will be great, but the company should give us some samples to try.
Yanlin, Staff Scientist, North America
The "next-generation" conjugated antibodies would have an impact on the technical inflexibility of molecular cell biology techniques, by providing further options in novel areas of protein characterization.
Catherine, Post Doctoral Fellow, Europe
The coupling of nanoparticles to antibody conjugates is a very interesting system of delivery. Antibodies against vital proteins can be driven to high metabolism cells, such as tumoral cells, where actually it's been successfully tested. One of the aspects of my research is to deliver drugs through the Blood Brain Barrier (BBB), to target HIV/SIV. Classically, though, antibodies do not cross this pathway. If the nanoparticles will turn out to induce Abs to cross the BBB, that has to be tested. However, if that happens, we also have to worry the consequences of the immuno-complex formation in the brain. By the other hand, that new generation may be useful in experimental systems to label molecules during the metabolic process. That may open a whole window of possibilities and interesting results.
M.Cecilia, Staff Scientist, North America
The implementation of "next-generation" conjugated antibodies which offer greater sensitivity accompanied by reduced background and interference hopefully will improve detection capabilities while providing for enhanced signal-to-noise ratios.
Rom, Lab Director/Supervisor/Coordinator, Africa
The nanoparticle revolution should be extremely interesting in my field as the use of these new antibodies will allow us to combine immuno-fluorescence, which is easy but still relatively low resolution, with the accuracy of electron microscopy. This will allow us to perform simply much more exact experiments than are currently possible. In addition to this when these products, such as quantum dots, are utilized with reagents such as FLASH and REASH and technologies like FRET I personally believe a fantastic new tool is available to molecular and cellular biologists.
Matthew, Graduate Student/Research Assistant, Europe
The new Q-dot technology offers an exciting and flexible way to study multiple proteins and complexes in vivo at the same time. By preconjugating antibodies to Q-dots, mixing together and using something like the Chariot reagent, we can introduce large amounts and multiple types of antibodies to study localization and complex formation of these proteins in real time (in vivo). It would be a new and elegant way of deciphering exact roles of complexes and complex components under different varieties of conditions/environments/treatments. This would help my research as I look at alternative splicing-dependent signal transduction and recruitment of complexes to the cellular membrane in neuronal development. Having a method to study and measure this complex formation instead of using the traditional (and limiting) GFP/DsRed/CFP/YFP methods would be of great benefit to my research.
Sachin, Graduate Student/Research Assistant, North America
The next generation antibodies will expedite the experiments with better results in form of specificity, quality and low background.
Shipra, Post Doctoral Fellow, North America
The next generation conjugated antibodies coupled to nanoparticles would pave the way to much more accuracy in antigen detection and to the design of new experiments at the subcellular organelle level. A new era in cell biology should start soon.
Dominique, Lab Director/Supervisor/Coordinator, Europe
The next generation of conjugated antibodies will allow us to improve current applications and usher into other avenues that are otherwise impossible.
Armah, Principal Investigator, North America
The next generation of conjugated antibodies will extremely aid my research. We have come to the point where we are trying to characterize the expression of surface markers and factors produced by specific subpopulation of immune cells. The second generation of antibodies will allow us to focus down to that important subpopulation of cells under conditions that involve an intricate interaction between different cell populations, e.g. seeing if in the population of T cells activated by certain antigen presenting cells are regulatory T cells.
Andrew, Principal Investigator, North America
The next-generation conjugated antibodies will be most useful in research on the effectiveness and bioavailabilty of growth factors and related agents in osteogenesis as related to skeletal repair materials, in particular, carbonated apatite cements.
Allan, Department Head, North America
The next-generation conjugated antibodies will definitely open new areas in research especially cancer research and I hope many unanswered questions will be answered in future.
Smriti, Lab Director/Supervisor/Coordinator, Asia
The performance of the nanoparticles should be better than current day techniques. I hope that nanoparticles will provide an opportunity to multiplex antibody-based assays.
Helen, Lab Director/Supervisor/Coordinator, North America
The prices of pre-made antibodies are generally too expensive for screening experiments. Dropping the prices somewhere around $150/vial will be much more competitive.
Mengfeng, Principal Investigator, North America
The sensitivity could possibly become better when using the "next-generation" of antibodies. This would have the advantage that less protein (and less antibody) is necessary for each experiments which is important for experiments with low sample sizes. But I am in doubt whether the specificity of the antibodies will be improved. And that is the most important need !
Wolfgang, Principal Investigator, Europe
The simpler handling and greater stability of nanoparticle conjugates, plus the higher detection sensitivity is a big help, as long as prices are reasonable.
Bernardo, Graduate Student/Research Assistant, Asia
The use new antibodies conjugated would be applied in investigations of illnesses of the nervous system during the development, to be able to understand the mechanisms of as develop the illnesses as Parkinson diseases and others.
J. Carlos, Graduate Student/Research Assistant, Central/South America
The use of antibodies coupled to infrared dyes would be extremely valuable as it would provide quicker, more quantitative and long term storage for western blots.
Esra, Post Doctoral Fellow, North America
The use of antibodies in research could be divided into two general categories, detection and selection. For the purpose of selection a capture method, typically protein A bead type, is often a source of background or contamination that is a technical setback. Antibodies or antibody conjugates coupled to nanoparticles could represent technical progress in this important step of protein selection.
Anthony, Staff Scientist, North America
The use of conjugated antibodies as primary antibody.
Xiao-Lu, Staff Scientist, North America
The use of next generation conjugated antibodies will I think make the whole procedure more reliable and valid. We very often hear about people using the same antibody in different concentrations and it does work for everybody in their own experiments, but if I try to reduce the amount of the antibody added just because the lower concentration worked for somebody else, it might not work for me. Also sometimes the same antibody starts behaving differently after you have kept it in the refrigerator for a while, even thought the cold chain was maintained. The affinity of the protein for the antibody is also different for different proteins. so for all these reasons the results are often not the same if the same experiment is repeated with the same antibodies. I guess these shortcomings will be removed with the advent of the next generations Abs.
Tarandeep, Graduate Student/Research Assistant, North America
The use of 'next-generation' conjugated antibodies will allow me to correlate directly my immunofluorescences observations with electron microscopic observations.
John, Professor/Teacher, North America
The use of next-generation conjugated antibodies will help my research by simplifying methods as a result of the nanoparticles.
Kim, Graduate Student/Research Assistant, North America
The use will greatly help my research.
John, Staff Scientist, North America
There is a great potential for the enzyme conjugated antibodies to be used as specific catalyst in complex substrate mixtures. Robustness is going to be the limiting factor.
Jon, Staff Scientist, North America
There is a need for different types of pre-conjugated anti-cytokine and anti-immunoproteins Ab needed for use in the ELISPot assay so that it is easier to evaluate multiple proteins in a single well.
Kimberly, Staff Scientist, North America
These antibodies could help to shape the future of diagnostic testing and improve patient care.
Alicia, Staff Scientist, North America
These antibodies will increase the through-put of my assays.
Yaron, Staff Scientist, North America
These conjugated antibodies could potentially be of great value in increasing the sensitivity and robustness of immunoassays if they could be used as a detection system to increase the sensitivity with low background instead of the currently available AlkPhos-MUP or HRP-TMB detection systems.
John, Staff Scientist, North America
These current technologies will improve sensitivity of the detection that allow us to apply antibodies on wider field. However increment of sensitivity directly links to higher background and pseudopositive signals. In parallel to the technology suppliers should be much more careful for the quality control of their products.
Masumi, Professor/Teacher, North America
These kinds of antibodies should help me to improve the protein cell immunolocalization.
Ignacio, Staff Scientist, Central/South America
These may help accelerate or will have no effect to our research depending on price, availability, reliability and equipment required.
George, Professor/Teacher, Europe
These next generation antibodies would help us in determining protein function in animal and human tissues and our tissue / protein microarray studies.
Ali, Professor/Teacher, Europe
These would be useful if they improved the sensitivity and multiplexing capability of the assays.
Robert, Staff Scientist, North America
These would permit significantly enhanced structure/ function mapping: are proteins that "seem" to co-localize using available reagents, really in the same anatomic structure/region, and therefore likely to interact with one another?
David, Professor/Teacher, North America
These would serve to advance studies of protein delivery and specificity. Magnetic nanoparticles like this would be interesting.
Guy, Principal Investigator, North America
They could be used for applications including cell sorting.
Heather, Graduate Student/Research Assistant, Europe
They may help in modeling drug delivery systems.
Alan, Lab Director/Supervisor/Coordinator, North America
They will be very important for immunofluorescence.
Alfonso, Post Doctoral Fellow, Europe
They will dramatically help research. You may use it to track the changes of target protein in living cells and can deliver the specific ab to the right sites of the cells or tissues. Even these can be used to treat disease more specifically.
Li, Principal Investigator, North America
They will increase the affinity of the antibodies.
Willias, Post Doctoral Fellow, Europe
They would definitely help with AB's for steroids with similar structures.
Todd, Laboratory Technician, North America
They would enhance subcellular localization of proteins at the light microscopic level, facilitating detection of changes in cellular localization under different physiological and clinical conditions.
Konrad, Professor/Teacher, North America
They would make things a lot easier and more accurate.
Sarah, Staff Scientist, North America
They would serve as a complement to our current studies.
Anthony, Lab Director/Supervisor/Coordinator, North America
This is definitely an issue for electron microscopy — it will spur the field of microcytochemistry (nanocytochemistry?)
Michel, Lab Director/Supervisor/Coordinator, Europe
This would enhance research. Nanoparticles have wider range of detection, better surface area, more efficient delivery.
Beverly, Principal Investigator, North America
This would increase the value of my work thru increasing output, efficiency and sensitivity.
Sonali, Lab Director/Supervisor/Coordinator, North America
Though I am generally curious about new reagents and applications, this of course will depend on what you can do with them. Right now, I do not have any clues about the benefits of nanoparticles except as a DNA carrier for shotgun transfections and magnetic beads for affinity purposes. So when there is an interesting application or functionality that might promote my research — why not?
Wolfgang, Post Doctoral Fellow, Europe
Though I am not familiar with the technological advantages, if these conjugates would be as easy or easier to use when compared to other conjugated antibodies in the application of hybridoma generation and screening, I would definitely give them a try!
Kim, Staff Scientist, North America
To carry out fluorescence resonance energy transfer (FRET) studies and single molecule tracking of cell surface receptors. To study single molecule dynamics in cells on a nanoscale for lifetime imaging.
Anu, Staff Scientist, North America
To determine pathways that are important for repair of cells especially neurons.
Farida, Principal Investigator, North America
To establish microbial succession in fish larval systems.
Somnath, Graduate Student/Research Assistant, Asia
To identify selectively upregulated or downregulated immunological markers during and before administration of HIV vaccines to HIV— negative vaccines compared to HIV infected vaccines on HAART and without HAART.
Beatrice, Staff Scientist, Africa
To impress journal reviewers.
Andrew, Post Doctoral Fellow, Europe
To increase the sensitivity and specificity of my assays and perhaps to use antibodies as a vehicle to deliver nanoparticles to specific sites.
Collins, Professor/Teacher, North America
Ultrasensitive immunoassay methods are used in clinical diagnostics to measure extremely low concentrations of specific compounds in highly complex samples. New detection methods based on high specific-activity particulate labels, such as fluorescent nanoparticles, are used today as a part of clinical diagnostics and biological, genomic, and pharmaceutical research. These submicrometer-sized labels are coupled to specific binding reagents such as nucleic acid probes, receptors, lectins, enzymes, and antibodies to detect specific molecules with sensitivities equal to or better than the best conventional labels available. Our interest in new label technologies has increased because none of the commonly used direct or enzyme-amplified radioactive, colorimetric, luminescent, or fluorescent reporter molecules fulfills all of the requirements for an ideal label, including sensitivity, specific activity, size, nontoxicity, stability, reliability, localization, detection and cost.
Geoffrey, Staff Scientist, North America
Use of antibody conjugates coupled to nanoparticles could potentially provide the advantage of more specifically targeting cellular proteins in vivo without the major disruptions caused by antibodies.
Jani, Professor/Teacher, North America
Use of new antibody conjugates should help high throughput applications, with better reproducibility, and less time.
Kevin, Staff Scientist, North America
Useful in relationship to cheaper and more efficient testing on a molecular level.
Sandra L., Department Head, North America
Utilization of antibodies in protein research, in particular proteomics, will increase dramatically. Thus, prices, antibody specificity, customer service, technical information, and availability of resources are extremely important for scientists.
Mamoun, Post Doctoral Fellow, North America
Very frequently, since that will give a window for understanding more about many difficult diseases and also the protein functions etc.
Idress, Graduate Student/Research Assistant, Europe
Very helpful. But currently, they all have problems with low density.
Haiyan, Post Doctoral Fellow, North America
Very much!!
Mauricio, Principal Investigator, North America
Very often antibodies are only tested against mammalian cells despite data, such as PubMed citations, indicating that vast numbers of scientists are studying specific antibody-relevant processes in other model systems (invertebrates, etc.). As an invertebrate model organism researcher, I am often tempted to try these non-tested antibodies yet the companies are not willing to send a small trial aliquot. Most often they are willing to refund my money if the product does not work, but the idea of spending precious research hours recouping costs is not worth it to me.
Kim, Principal Investigator, North America
Visualization of cellular uptake and transport processes.
Emil, Principal Investigator, North America
We are doing study at single cell level, so in that kind of study, the nanoparticle coupled antibody will be very helpful.
Manoj, Staff Scientist, North America
We are hopeful that nanoparticle labeling of antibodies will allow very high resolution intracellular localization of signaling molecules.
Stanley, Principal Investigator, North America
We are very excited about the use of nanoparticles, our initial tests show them to work very well indeed. We await the flexibility that a range of different secondary antibodies will bring to our research. I expect the first 6 color labeling will appear soon!!!!
Mike, Lab Director/Supervisor/Coordinator, Europe
We haven't thought to move in that direction but if our customers start to use it, so will we.
Amy, Staff Scientist, North America
We mostly do basic research so I don't think "next-gen" antibodies are what we're looking for, yet.
Christopher, Post Doctoral Fellow, Europe
We would probably use them if they were affordable.
Guy, Principal Investigator, Europe
We would use the next generation antibodies for cell/protein trafficking within living cells using time-lapsed photography to follow our proteins of interest.
Mary, Principal Investigator, North America
Western Blotting and ELISAs are in my opinion the most common way to quantify protein levels. In basic science, it would seem that the Western Blot is the most commonly used form. The issue with this is the time investment to run/transfer/probe gels. Using nanotechnology it may be possible to create cell lysates and then measure the levels of a specific protein in that lysate directly. This will save an inordinate amount of time both in the lab, in the clinic, and possibly in the field to detect bioterrorism materials.
Eric, Post Doctoral Fellow, North America
Will not help at all.
Alexandre, Staff Scientist, North America
With the explosion of mouse based research, the use of mouse monoclonals on mouse tissue is necessary yet problematic. The non-specific binding of secondaries could be circumvented if companies would offer their primaries conjugated to quality fluorophores (like the Alexa fluors). In an ideals world, every antibody would be available with a choice of conjugate.
Helen, Principal Investigator, North America
Would be very helpful provided they do not cross-react, and I experience lower backgrounds and higher sensitivity.
Janos, Graduate Student/Research Assistant, North America
Would be very useful for our drug targeting studies in animals and in cultured cells.
Ibrahim, Post Doctoral Fellow, North America
Would enable me to detect low-concentration virus antigens/proteins in infected cells/tissue explants.
Karen, Principal Investigator, North America
Yes, we are looking for new type of antibodies. The nanoparticle-antibody is very useful as it can not only bind to its target but also deliver chemicals to the target molecules.
Yong-jian, Lab Director/Supervisor/Coordinator, North America
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